Yale School of Public Health 1 article published in JoVE Biology Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus Hae-Na Chung1, Stacy D. Rodriguez1, Victoria K. Carpenter2, Julia Vulcan1, C. Donovan Bailey1, Madhugiri Nageswara-Rao1, Yiyi Li3, Geoffrey M. Attardo4, Immo A. Hansen1,5 1Department of Biology, New Mexico State University, 2Department of Molecular Genetics and Microbiology, Duke University, 3Department of Computer Sciences, New Mexico State University, 4Department of Epidemiology of Microbial Diseases, Yale School of Public Health, 5Institute of Applied Biosciences, New Mexico State University The fat body is the central metabolic organ in insects. We present a live organ culture system that enables the user to study the responses of isolated fat body tissue to various stimuli.