University of Georgia Cooperative Extension 1 article published in JoVE Environment Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification Owen Hudson1, Sumyya Waliullah1, Justin Hand2, Romina Gazis-Seregina3, Fulya Baysal-Gurel4, Md Emran Ali1 1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.
