Corteva Agriscience 1 article published in JoVE Biology Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes Alicia Masters1,2, Minjeong Kang1,3,4, Morgan McCaw1,3, Jacob D. Zobrist1,3,5, William Gordon-Kamm2, Todd Jones2, Kan Wang1,3 1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.
