The Ragon Institute of MGH, MIT and Harvard 1 article published in JoVE Immunology and Infection In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function Filippos Porichis*1, Meghan G. Hart*1, Jennifer Zupkosky1, Lucie Barblu1,2, Daniel E. Kaufmann1,2 1The Ragon Institute of MGH, MIT and Harvard, 2 We developed an in vitro assay to investigate the role of immunoregulatory pathways in the regulation of cytokine secretion by HIV-specific CD4 T cells.