Andor Technology an Oxford Instruments Company 1 article published in JoVE Bioengineering A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging Jialei Tang1, Chun-Hung Weng1, Jeffrey B. Oleske2, Kyu Young Han1 1CREOL, The College of Optics and Photonics, University of Central Florida, 2Andor Technology, Oxford Instruments A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.