University of Minnesota, College of Veterinary Medicine 1 article published in JoVE Cancer Research Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures John P. Zepecki1, Kristin M. Snyder2, Nikos Tapinos1,3 1Molecular Neuro-oncology Laboratory, Brown University, Rhode Island Hospital, 2University of Minnesota, College of Veterinary Medicine, 3Department of Neurosurgery, Brown University Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.