Curia Global Inc. 1 article published in JoVE Biochemistry Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System James B. Pace1, Nian N. Huang1, Jacqueline P. Séguin1, Candi Esquina1, Erin Olin1, Guangyu Zhu1, Grant Carr1 1Discovery Biology, Curia Global Inc. We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.
