State University of New York at Buffalo 1 article published in JoVE Biology Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies Oluwatoyin Campbell1, Van Le2, Angela Aguirre1, Viviana Monje-Galvan1 1Department of Chemical and Biological Engineering, State University of New York at Buffalo, 2Department of Mathematics, State University of New York at Buffalo Membrane lipid diversity in structure and composition is an important contributor to cellular processes and can be a marker of disease. Molecular dynamics simulations allow us to study membranes and their interactions with biomolecules at atomistic resolution. Here, we provide a protocol to build, run, and analyze complex membrane systems.