Miami University View Institution's Website 5 articles published in JoVE Engineering Measurement of the Hand Transmitted Vibration of the Human Hand Arm System During Operation of a Hand Tractor Shiqing Lu1, Rui Jiang2, Xinyi Xiao3, Yong Li1, Xia Huang1, Kun Song1, Changming Chen1, Jun Ding1 1School of Mechanical Engineering, Chongqing University of Technology, 2College of Mechanical and Electrical Engineering, Wenzhou University, 3Mechanical and Manufacturing Engineering, Miami University Here, we present a standardized method for measurement of the hand transmitted vibration from handles of a single-axle tractor with special reference to changes in grip force and vibration frequency. Environment RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points Shubham Rathore1, Jenni Hassert1, Courtney M. Clark-Hachtel2,3, Aaron Stahl1,4, Yoshinori Tomoyasu2, Elke K. Bushbeck1 1Department of Biological Sciences, University of Cincinnati, 2Department of Biology, Miami University, 3Department of Biology, University of North Carolina at Chapel Hill, 4Department of Neuroscience, The Scripps Research Institute RNA interference is a widely applicable, powerful technique for manipulating gene expression at specific developmental stages. Here, we describe the necessary steps for implementing this technique in the aquatic diving beetle Thermonectus marmoratus, from the acquisition of gene sequences to the knockdown of genes that affect structure or behavior. Medicine Dissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling Michelle J. Veite-Schmahl1, Daniel P. Regan1, Adam C. Rivers1, Joseph F. Nowatzke1,2, Michael A. Kennedy1 1Department of Chemistry & Biochemistry, Miami University, 2College of Medicine, Central Michigan University This video article provides a detailed demonstration of the procedures required to successfully remove the pancreas from a mouse by dissection for histological analysis and metabolic profiling. Biology Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum David M. Linz*1, Courtney M. Clark-Hachtel*1, Ferran Borràs-Castells*1, Yoshinori Tomoyasu1 1Department of Biology, Miami University RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts. Biology Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential Jenna M. Dolhi1, Nicholas Ketchum1, Rachael M. Morgan-Kiss1 1Department of Microbiology, Miami University Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.