Case Western Reserve University School of Medicine 5 articles published in JoVE Engineering The Immersive Cleveland Clinic Virtual Reality Shopping Platform for the Assessment of Instrumental Activities of Daily Living Jay L. Alberts1,2, Morgan McGrath1,3, Mandy Miller Koop1, Colin Waltz1, Logan Scelina1, Kathryn Scelina1, Anson B. Rosenfeldt1 1Department of Biomedical Engineering, Cleveland Clinic, Lerner Research Institute, 2Neurological Institute, Center for Neurological Restoration, Cleveland Clinic, 3Case Western Reserve University, School of Medicine Virtual reality (VR) is a powerful yet underutilized approach to advance the diagnosis and treatment of neurological disease. The Cleveland Clinic Virtual Reality Shopping platform combines state-of-the-art VR content with an omnidirectional treadmill to quantify instrumental activities of daily living-a proposed prodromal marker of neurological disease. Cancer Research Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells Raffaella Spina1, Dillon M. Voss1, Laura Asnaghi2, Andrew Sloan1,3, Eli E. Bar1 1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine An efficient screening protocol is presented for the identification of small molecules that promote astroglial differentiation in glioblastoma stem cells (GSCs). The assay is based on a stem cell differentiation reporter whereby the expression of the enhanced GFP (eGFP) is driven by the human GFAP promoter. Biochemistry Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro Ryan W. Clinton1, Jason A. Mears1 1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology, Case Western Reserve University School of Medicine This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment. Medicine Ferric Chloride-induced Murine Thrombosis Models Wei Li1,2, Marvin Nieman3, Anirban Sen Gupta4 1Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 2Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, 3Department of Pharmacology, Case Western Reserve University, 4Department of Biomedical Engineering, Case Western Reserve University We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation. Medicine Flexible Colonoscopy in Mice to Evaluate the Severity of Colitis and Colorectal Tumors Using a Validated Endoscopic Scoring System Tomohiro Kodani*1,3, Alex Rodriguez-Palacios*1,3, Daniele Corridoni1,3, Loris Lopetuso2,3, Luca Di Martino1,3, Brian Marks1,3, James Pizarro1,3, Theresa Pizarro2,3, Amitabh Chak1,3, Fabio Cominelli1,3 1Division of Gastroenterology, Case Western Reserve University School of Medicine, Cleveland, 2Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, 3Digestive Health Research Center, Case Western Reserve University School of Medicine, Cleveland Over the past few years, new generation endoscopes have emerged as important diagnostic research aids for evaluating murine colitis and colorectal tumors. We present herein a detailed protocol for endoscopic assessment of inflammation and colorectal tumors in mice, as well as a novel scoring system that uses decimal identifiers to document the endoscopic severity of colitis and colorectal tumors.