Nanofiber Solutions, Inc 1 article published in JoVE Bioengineering Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model Matthew G. Wiet*1,2, Sayali Dharmadhikari*1,3, Audrey White1,2, Susan D. Reynolds4, Jed Johnson5, Christopher K. Breuer3,6, Tendy Chiang1,3 1 Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.