Oklahoma State University Center for Health Sciences 2 articles published in JoVE Medicine Laryngeal Mask Airway (LMA) Placement in a Neonatal Patient Simulator Using a Non-Inflatable Supraglottic Airway (SGA) Birju A. Shah1,2,3, Michelle Blunt3, Netsanet Kassa2, Doug Dannaway1 1University of Oklahoma (OU) Health Sciences Center, 2 This protocol is designed to describe a method to place and establish a supraglottic (alternative) airway during neonatal resuscitation. It can be used in situations where frontline providers cannot ventilate or endotracheally intubate neonates requiring resuscitation. Immunology and Infection Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein Vikramsingh Gujar*1, Michael B. Anderson*1, Kenneth E. Miller*1, Radhika D. Pande1, Pranav Nawani2, Subhas Das1 1Department of Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics, Washington State University Presented here is a protocol for the separation of epidermis from dermis to evaluate inflammatory mediator production. Following inflammation, rat hind paw epidermis is separated from the dermis by thermolysin at 4 °C. The epidermis is then used for mRNA analysis by RT-PCR and protein evaluation by western blot and immunohistochemistry.
