University of Mary Hardin-Baylor 1 article published in JoVE Developmental Biology Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions Joni H. Ylostalo1, Nikolay Bazhanov2, Arezoo Mohammadipoor3, Thomas J. Bartosh4 1Department of Biology, University of Mary Hardin-Baylor, 2Department of Pediatrics, University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, 4Internal Medicine, Texas A&M University Health Science Center The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
