Universidad Central del Caribe 1 article published in JoVE Neuroscience Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions Yancy Ferrer-Acosta*1, Maxine N. Gonzalez-Vega*1, David E. Rivera-Aponte2, Solianne M. Martinez-Jimenez1, Antonio H. Martins3 1Department of Neuroscience, School of Medicine, Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine, Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus, University of Puerto Rico Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.
