Universidad del Bio-Bio 1 article published in JoVE Medicine Disruption of the Mouse Blood-Brain Barrier by Small Extracellular Vesicles from Hypoxic Human Placentas Hermes Sandoval*1, José León*1,2, Felipe Troncoso1, Valeria de la Hoz3, Aaron Cisterna3,4, Moisés Contreras1, Fidel O. Castro5, Belén Ibañez1, Jesenia Acurio1, Carlos Escudero1,6 1Vascular Physiology Laboratory, Department of Basic Sciences, Universidad del Bío-Bío, Chillan, 2Nursery School, Health Faculty, Universidad Santo Tomás, Los Angeles, 3Obstetrics and Gynecology Department, Herminda Martin Clinical Hospital, Chillan, 4Faculty of Medicine, Universidad Católica de la Santísima Concepción, Concepcion, 5Laboratory of Animal Biotechnology, Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Chillan, 6Group of Research and Innovation in Vascular Health (GRIVAS Health), Chillan A protocol is presented to evaluate whether small EVs (sEVs) isolated from placental explants cultured under hypoxic conditions (modeling one aspect of preeclampsia) disrupt the blood-brain barrier in nonpregnant adult female mice.
