Universidad Espiritu Santo 1 article published in JoVE Biology Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria Jorge Olivares1, Annia González1, Derly Andrade1,2, Mónica Vásquez1 1Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 2Laboratorio de Ciencias Omicas, Facultad de Ciencias de la Salud, Universidad Espíritu Santo We present a simple and accessible method for filamentous cyanobacterial visualization in the XY plane. A low-melting point agarose matrix was used, allowing the acquisition of images of proteins involved in the division, in a vertical orientation. Therefore, this methodology can be applied to any filamentous organism and different kinds of proteins.
