Materials
| Name | Company | Catalog Number | Comments |
| 10x MEM | GIBCO, by Life Technologies | 21430 | - |
| NaOH | Sigma-Aldrich | 367176-500G | Prepare 0.22 M stock in water |
| FCS | PAA Laboratories | A15-101 | - |
| 35 mm dishes | Falcon BD | 353001 | For step 3.4 |
| 60 mm dishes | Falcon BD | 353004 | For step 5.2 |
| Spring forceps blunt | Samco Scientific, Thermo Fisher Scientific | E003/02 | Toothed, not smooth |
| 16% paraformaldehyde | Electron Microscopy Sciences | 15710 | Dilute to 4% in PBS prior to use |
| Screens for cd-1, size 40 mesh | Sigma-Aldrich | S07707-5EA | Stainless steel grids, 5 per pack |
| Dialysis tubing | Medicell International | 7607 2295 | 12 - 14 kD |
| PBS | Oxford Labware | BR0014G | - |
| Acetic Acid | Sigma-Aldrich | 242853 | - |
| 24 well dish | Falcon BD | 353047 | For step 4.1 |
| Fungizone | Invitrogen | 15290018 | - |
References
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