Protocol
Assessment and Communication for People with Disorders of Consciousness
Rupert Ortner1, Brendan Z. Allison1, Gerald Pichler2, Alexander Heilinger3, Nikolaus Sabathiel1, Christoph Guger1,3
1Guger Technologies OG, 2Albert Schweitzer Hospital Graz, 3g.tec Medical Engineering GmbH
With this experiment, one might be able to detect consciousness in people with disorders of consciousness. Furthermore, the approach can create a simple communication channel that enables people to give simple YES/NO answers to questions.
Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
Eric S. Michel1,2, Emily B. Flinn1, Stephen Demarais1, Bronson K. Strickland1, Guiming Wang1, Chad M. Dacus3
1Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, 2Department of Natural Resource Management, South Dakota State University, 3Mississippi Department of Wildlife, Fisheries and Parks
Phenotypic differences among cervid populations may be related to population-level genetics or nutrition; discerning which is difficult in the wild. This protocol describes how we designed a controlled study where nutritional variation was eliminated. We found that phenotypic variation of male white-tailed deer was more limited by nutrition than genetics.
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
Antionette L. Williams, Brenda L. Bohnsack
Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan
A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Yu Shrike Zhang1, Qingmeng Pi1,2, Anne Metje van Genderen1,3
1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Department of Plastic and Reconstructive Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 3Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
Disclosures
No conflicts of interest declared.
