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Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Article DOI: 10.3791/1120-v • 13:37 min • March 27th, 2009
March 27th, 2009 •
1, 1, 1, 1
1Unitat Biologia Cellular (Facultat de Biociències), Universitat Autonoma de Barcelona

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Summary
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In this video-article we present a step-by-step demonstration on how to collect and cryopreserve hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.

Tags

Cryopreservation Hamster Oocytes Mouse Embryos Slow Freezing Vitrification Cryoprotectants Ice-crystal Formation Solution Effects Osmotic Shock Cryoinjuries Programmable Freezers Supercooling Effects
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