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JoVE Journal Biology
Making Gynogenetic Diploid Zebrafish by Early Pressure
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Making Gynogenetic Diploid Zebrafish by Early Pressure

Article DOI: 10.3791/1396
June 30th, 2009 •
1, 2, 2
1Institute of Neuroscience, University of Oregon, 2Division of Basic Science, Fred Hutchinson Cancer Research Center - FHCRC

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June 30th, 2009

This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.

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