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Aislamiento y trasplante de células madre hematopoyéticas (HSC)
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JoVE Journal
Biology
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
Please note that all translations are automatically generated.
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Aislamiento y trasplante de células madre hematopoyéticas (HSC)
DOI:
10.3791/157-v
•
20:38 min
•
February 25, 2007
•
Cristina Lo Celso
,
David Scadden
1
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute
,
Harvard Medical School
Chapters
00:00
Introduction
00:20
Isolating bone marrow cells
00:21
Dissecting mouse to isolate bones
01:03
Isolating tibia
01:40
Isolating femur
02:12
Isolating hip bone
02:50
Isolating spine
04:07
Cleaning bones
06:37
Crushing bones
09:33
Lineage depletion of bone marrow cells
14:06
Antibody staining for FACS
16:06
Transplantation into irradiated mouse
18:50
Discussion
Tags
Isolation
Transplantation
Hematopoietic Stem Cells
HSCs
Article
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