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Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
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Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

Article DOI: 10.3791/1865-v • 09:35 min • March 30th, 2010
March 30th, 2010 •
1, 1
1Department of Plant Sciences, Tel Aviv University

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In this video, we describe a procedure for the expression of bacterial type III effectors in yeast and the identification of effector-induced growth inhibition phenotypes. Such phenotypes can be subsequently exploited to elucidate effector functions and targets.

Tags

Growth Inhibition Phenotypes Bacterial Type III Effectors Gram-negative Pathogenic Bacteria Type III Secretion System Effector Proteins Host Cells Immune Responses Pathogen Growth Effector Repertoire Virulence Infection Stages Genetic Manipulation Budding Yeast Saccharomyces Cerevisiae Functional Characterization Of Effector Proteins Cellular Processes Eukaryotes
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