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Quantitation of γH2AX Foci in Tissue Samples
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Quantitation of γH2AX Foci in Tissue Samples

Article DOI: 10.3791/2063-v • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

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Summary
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Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.

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Quantitation γH2AX Foci Tissue Samples DNA Double-strand Breaks Endogenous Processes Exogenous Factors Ionizing Radiation Radiomimetic Compounds H2A Histone Variant Serine-139 Residue Phosphorylation PI3K Family Of Proteins ATM Kinase ATR Kinase Nuclear γH2AX Foci Immunofluorescence Microscopy Marker Specificity And Sensitivity Biomedical Research Radiation Biology Nuclear Medicine Cell Culture Systems Ionizing Radiation-induced DSBs Cellular Radiosensitivity Radiation-modifying Compounds DSB-inducing Compounds
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