Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / An Assay for Measuring the Activity of…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
Journal (Biology)
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
Journal (Biology)
The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
 

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

Article DOI: 10.3791/2094-v • 08:09 min • December 19th, 2010
December 19th, 2010 •
1, 1
1Department of Biochemistry, University of Toronto

Chapters

Summary
Automatic Translation

The activity of the inducible lysine decarboxylase is monitored by reacting the substrate L-lysine and the product cadaverine with 2,4,6-trinitrobenzensulfonic acid to form adducts that have differential solubility in toluene.

Tags

Assay Measuring Activity Escherichia Coli Inducible Lysine Decarboxyase PH Acidic PH Survival Amino Acid Decarboxylases Glutamic Acid Decarboxylases Arginine Decarboxylase Lysine Decarboxylase Ornithine Decarboxylase Pyridoxal-5'-phosphate Co-factor Inner-membrane Substrate-product Antiporters Lysine-cadaverine Antiporter CadB X-ray Crystal Structure Stringent Response Regulator Guanosine 5'-diphosphate 3'-diphosphate PpGpp Stringent Response
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter