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Biology
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维持细胞培养基的Wolbachia
JoVE Journal
Biology
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JoVE Journal
Biology
Maintaining Wolbachia in Cell-free Medium
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Click here for the English version.
维持细胞培养基的Wolbachia
DOI:
10.3791/223-v
•
13:33 min
•
July 04, 2007
•
Courtney Gamston
,
Jason Rasgon
1
Malaria Research Institute, Bloomberg School of Public Health
,
Johns Hopkins University
Chapters
00:06
Introduction
01:24
Lysing Infected Anopheles Cells
03:34
Filtering Lysates and Isolation of Wolbacchia
09:31
Assay of Wolbacchia Viability Using Live/Dead Stain
12:11
Conclusion
13:22
End Credits
Summary
Automatic Translation
English (Original)
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Automatic Translation
本视频介绍了净化按蚊细胞系的Wolbachia pipientis,然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。
Tags
Wolbachia
Cell-free Medium
Video Protocol
Purify
Symbionts
Cultured Mosquito Cells
Fluorescent Assay
Viability
Extracellular Phase
Replicate
Eukaryotic Cells
Micro-injection
DNA Extraction
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