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JoVE Journal Neuroscience
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Article DOI: 10.3791/2457-v • 11:55 min • January 11th, 2011
January 11th, 2011 •
1,2, 2, 2, 2, 2
1Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 2Department of Neurosurgery, The University of Florida

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Summary
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This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

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Embryonic Mouse Neural Stem Cell Culture Neurosphere Assay Stem Cells Undifferentiated Cells Cell Genesis Tissue Renewal Aging Process Injury And Disease Neural Stem Cells (NSCs) Serum-free Culture System Isolation And Expansion Of NSCs Embryonic CNS In Vitro Expanded NSCs CNS Cell Types Reliable And Consistent Results E14 Mouse Embryos Brain Microdissection Ganglionic Eminences Single Cell Suspension NSA Culture Primary Neurospheres
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