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Journal / Biology / Purificação da Hsp104, um Disaggregase Protein
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Purificação da Hsp104, um Disaggregase Protein

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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Aqui, nós descrevemos um protocolo para a purificação de altamente ativa Hsp104, a AAA hexamérica + proteína de levedura, que acopla a hidrólise de ATP a desagregação de proteína. Este esquema explora uma construção His6 marcadas para a purificação de afinidade

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Biologia Molecular Edição 55 Neuroscience Hsp104 AAA + disaggregase choque térmico amilóide prion
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