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Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

Article DOI: 10.3791/3244-v • 13:42 min • October 17th, 2011
October 17th, 2011 •
*1, *2, 2, 2, 2
1Department of Chemistry, Hunter College, 2Department of Biochemistry, Albert Einstein College of Medicine
* These authors contributed equally

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Summary
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This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.

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Monitoring Equilibrium Changes RNA Structure Peroxidative Oxidative Hydroxyl Radical Footprinting RNA Molecules Genetic Information Tertiary Structures Biologic Role Regulator Binder Catalyst Nucleic Acids Phosphodiester Backbone Single Nucleotide Resolution Intermolecular Contact Surface Equilibrium Transitions Kinetic Transitions Solution Variable Time Probe Exposure Polymer Sampling Video Article Sample Preparation Mg(II)-mediated Folding Isotherms
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