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Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Summary
Automatic Translation

Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.

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Rotating Cell Culture Systems Human Cell Culture Human Trophoblast Cells Model Primary Cultures Explant Cultures Trophoblast Cell Lines Invasion Of Uterine Wall Remodeling Of Uterine Spiral Arteries Extravillous Trophoblast Cells Successful Establishment Of Pregnancy In Vitro Cell Culture Models Rotating Cell Culture System (RCCS) Morphological Properties Phenotypic Properties Functional Properties EVT-like Cell Lines Differentiation Of EVTs Gene Expression Matrix Metalloproteinases (MMPs) Trophoblast Differentiation Saint Georges Hospital Placental Cell Line-4 (SGHPL-4) RCCS Culture Vessel Three-dimensional Environment
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