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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Article DOI: 10.3791/3410-v • 13:38 min • January 27th, 2012
January 27th, 2012 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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Summary
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FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

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Kinase Phosphatase Cell Cycle Ratiometric FRETFörster Resonance Energy Transfer FRET-based Reporters CFP YFP Phosphorylation Conformation Change Distance Orientation FRET Efficiency Probes PKA PKB PKC PKD ERK JNK Cdk1 Aurora B Plk1 Stress Signaling Pathways Progression Through The Cell Cycle Unperturbed Growth Recovering From Stress Time-lapse Imaging Signal To Noise Ratio
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