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Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Article DOI: 10.3791/3545-v • 10:42 min • February 6th, 2012
February 6th, 2012 •
*1, *1, 1
1Department of Comparative Biology and Experimental Medicine, University of Calgary
* These authors contributed equally

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Summary
Automatic Translation

Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.

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Germ Cell Transplantation Testis Tissue Xenografting Mice Dr. Ralph Brinster University Of Pennsylvania Microinjection Seminiferous Tubules Spermatogenesis Sperm Production Donor-derived Bacterial β-galactosidase Gene Offspring Lumen Of The Seminiferous Tubules Basement Membrane Spermatogonia SSCs Stem Cell Niche Putative Spermatogonial Stem Cells Transgenic Animals Genetic Manipulation Sertoli Cell-germ Cell Interaction SSC Homing And Colonization Self-renewal And Differentiation
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