Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Detection of Toxin Translocation into the Host…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Journal (Immunology and Infection)
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Visualizing Dengue Virus through Alexa Fluor Labeling
Journal (Immunology and Infection)
Visualizing Dengue Virus through Alexa Fluor Labeling
 

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Article DOI: 10.3791/3686-v • 10:41 min • January 3rd, 2012
January 3rd, 2012 •
1, 1, 1, 1
1Department of Molecular Biology and Microbiology, University of Central Florida

Chapters

Summary
Automatic Translation

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

Tags

Toxin Translocation Surface Plasmon Resonance AB Toxins Cell-binding B Subunit Vesicle Carriers Endoplasmic Reticulum (ER) Catalytic A Chain Protein-conducting Channel Cytosolic Target Toxin Trafficking Lysosomes Golgi Apparatus Subcellular Fractionation Protocol Digitonin Permeabilization Anti-toxin A Chain Antibody Detection Method
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter