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Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
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Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Article DOI: 10.3791/3987-v • 08:01 min • May 30th, 2012
May 30th, 2012 •
1, 1
1Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences

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Summary
Automatic Translation

We describe a fluorescence reporter assay to quickly identify and characterize genes that regulate mouse embryonic stem cell maintenance and self-renewal.

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Oct4GiP Reporter Assay Genes Regulate Mouse Embryonic Stem Cell Maintenance Self-renewal Pluripotency Molecular Mechanism Developmental Biology Studies Disease Modeling Drug Discovery Regenerative Medicine Fluorescence Reporter-based Assay Self-renewal Status Mouse ES Cells Oct4 Gene Promoter Region Green Fluorescent Protein (GFP) Differentiation Fluorescence-activated Cell Sorting (FACS) Analysis RNAi Convenience Sensitivity Quantitative Analysis Cost-effective
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