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JoVE Journal Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
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Journal (Biology)
DNA Electroporation, Isolation and Imaging of Myofibers
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Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Article DOI: 10.3791/4254-v • 12:44 min • October 11th, 2012
October 11th, 2012 •
1, 1, 1
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

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Summary
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We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

Tags

LysoTracker Programmed Cell Death PCD Embryos Differentiating Embryonic Stem Cells Apoptosis Caspase Enzyme Cascade Membrane Blebbing Nuclear Shrinking DNA Fragmentation Autophagy Immune Cells Non-professional Phagocytes Lysosomal Activity
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