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Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

Article DOI: 10.3791/4286-v • 05:09 min • November 25th, 2012
November 25th, 2012 •
1,2, 3,4, 1, 1,3, 3,4
1Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, 2Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University, 3Omics Laboratory, University of Iowa, 4Department of Ophthalmology and Visual Sciences, University of Iowa

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Summary
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This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.

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Subretinal Injection Gene Therapy Vectors Stem Cells Perinatal Mouse Eye Blindness Retinal Degenerative Diseases Autologous Transplantation Age-related Macular Degeneration (AMD) Iris Pigment Epithelial Cell Transplantation RPE65 Gene Replacement Therapy Leber's Congenital Amaurosis RPE Cell Transplantation Stargardt's Disease Animal Models Therapeutic Efficacy Eye Tissue Preservation
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