Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / The Logic, Experimental Steps, and Potential of…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
Analyzing and Building Nucleic Acid Structures with 3DNA
Journal (Biology)
Analyzing and Building Nucleic Acid Structures with 3DNA
A Protocol for Computer-Based Protein Structure and Function Prediction
Journal (Biology)
A Protocol for Computer-Based Protein Structure and Function Prediction
 

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Article DOI: 10.3791/4346-v • 10:41 min • January 13th, 2013
January 13th, 2013 •
1, 2, 1
1Chemical and Biological Engineering Department, State University of New York at Buffalo, 2Chemical Engineering Department, Massachusetts Institute of Technology

Chapters

Summary
Automatic Translation

The heterologous biosynthesis of erythromycin A through E. coli includes the following experimental steps: 1) genetic transfer; 2) heterologous reconstitution; and 3) product analysis. Each step will be explained in the context of the motivation, potential, and challenges in producing therapeutic natural products using E. coli as a surrogate host.

Tags

Heterologous Natural Product Biosynthesis Complex Antibiotic Erythromycin A E. Coli Genetic Transfer Heterologous Reconstitution Product Analysis Biosynthetic Pathway Complexity Megasynthases PCR Isolation Operon Construction Multi-cystronic Plasmids Electro-transformation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter