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Genetic Modification and Recombination of Salivary Gland Organ Cultures

Article DOI: 10.3791/50060-v • 09:29 min • January 28th, 2013
January 28th, 2013 •
*1, *1, 1, 1
1Department of Biological Sciences, University at Albany, SUNY
* These authors contributed equally

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Summary
Automatic Translation

A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.

Tags

Genetic Modification Recombination Salivary Gland Organ Cultures Branching Morphogenesis Embryonic Mouse Submandibular Gland Epithelial Bud Duct Formation Complex Branched Network Acini Ducts Saliva Oral Cavity Basement Membrane Mesenchymal Compartment Mesenchyme Cells Growth Factors Extracellular Matrix Cellular Events Molecular Events Coordination Developmental Mechanisms Regenerative Medicine Approaches Genetic Manipulation Salivary Epithelium Adenoviral Transduction
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