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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Article DOI: 10.3791/50391-v • 10:39 min • July 5th, 2013
July 5th, 2013 •
1, 1, 1, 1, 2, 1
1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, 2Department of Surgery, Tufts University Medical Center

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Summary
Automatic Translation

We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.

Tags

Pancreatic Acinar Cells Calcium Imaging Cell Injury Measurements Adenoviral Infection Isolation Of Acinar Cells Intracellular Calcium Signals Transfection Adenoviral Constructs Lactate Dehydrogenase Leakage Acinar Cell Physiology Acinar Cell Pathology
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