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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

Article DOI: 10.3791/50399-v • 15:23 min • October 1st, 2013
October 1st, 2013 •
1, 1, 1
1Department of Biology, The University of Mississippi

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Summary
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Microplate based procedures are described for the colorimetric or fluorometric analysis of extracellular enzyme activity. These procedures allow for the rapid assay of such activity in large numbers of environmental samples within a manageable time frame.

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Microbial Extracellular Enzyme Activity High Throughput Microplate Assays Nutrient Cycling Carbon Processing Natural Environments Ecosystem Level Processes Organic Matter Decomposition Nitrogen Mineralization Phosphorus Mineralization Artificial Colorimetric Substrates Fluorometric Substrates Substrate Hydrolysis Microplate Based Methods 96-well Microplates Deep Well Microplate Blocks Absorption Fluorescence Microplate Reader Fluorometer High Throughput Procedures Spatially Separate Sites Cost Reduction
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