Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / En kvantitativ analyse til at studere Protein:…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
En kvantitativ analyse til at studere Protein
Human ES cells: Starting Culture from Frozen Cells
Journal (Biology)
Human ES cells: Starting Culture from Frozen Cells
A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
Journal (Biology)
A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
 
Click here for the English version

En kvantitativ analyse til at studere Protein: DNA interaktioner, Discover transskriptionsregulatorer af genekspression, og identificere nye anti-tumor Agents

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi har udviklet en kvantitativ DNA-bindende ELISA-assay til måling af transkriptionsfaktor interaktion med DNA. Høj specificitet for RUNX2 protein blev opnået med en konsensus DNA-genkendelse oligonukleotid og specifikt monoklonalt antistof. Kolorimetrisk detektion med et enzym-koblet antistof substrat Reaktionen blev overvåget i realtid.

Tags

Cellebiologi transkriptionsfaktorer D-vitamin Drug Discovery enzymmaerket Assay (ELISA) DNA-bindende transskription faktor narkotika screening antistof
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter