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JoVE Journal Biology
Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
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Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Article DOI: 10.3791/50717-v • 16:41 min • November 5th, 2013
November 5th, 2013 •
1, 1
1Department of Biochemistry, University of Geneva

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Summary
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We adapted a set of protocols for the measurement of reactive oxygen species (ROS) that can be applied in various amoeba and mammalian cellular models for qualitative and quantitative studies.

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Detecting Visualizing Quantitating Generation Reactive Oxygen Species Amoeba Model System ROS Oxygen-containing Molecules Protocols OxyBurst Green (OBG)-coated Beads Dihydroethidium (DHE) Amplex UltraRed (AUR) Monitor Qualitatively Quantitatively Professional Phagocytes Dictyostelium Lipopolysaccharide (LPS) E. Coli Intraphagosomal ROS Generation Single Cell Level Real Time Assays Medium-throughput Assays Intracellular Superoxide Production Extracellular H2O2 Production
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