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JoVE Journal Neuroscience
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Article DOI: 10.3791/50861-v • 11:10 min • November 27th, 2013
November 27th, 2013 •
1, 2, 1
1Department of Pharmacology and Physiology, Rutgers New Jersey Medical School, 2Center for Taste and Feeding Behavior, Universite de Bourgogne

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Summary
Automatic Translation

The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.

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Membrane Potential Dye Imaging Ventromedial Hypothalamus Neurons Adult Mice Glucose Sensing Neuronal Activity Connective Tissue Neuronal Viability Disease Models Fluorescence Imaging Electrophysiology Heterogeneous Neuronal Population Hypothalamic Glucose Sensing Neurons Extracellular Glucose Central Regulation Of Energy Balance Obesity
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