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JoVE Journal Neuroscience
Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
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Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals

Article DOI: 10.3791/50884-v • 09:53 min • February 11th, 2014
February 11th, 2014 •
1, 1, 1, 1
1CGphiMC, UMR CNRS 5534, University of Lyon 1

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Summary
Automatic Translation

The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.

Tags

Culture Isolated Floor Plate Tissue Production Conditioned Medium Functional Properties Floor Plate-released Signals Development Progenitors Post-mitotic Neurons Adjacent Territories Fate Regulation Glial Cells Ependymal Canal Ventral Position Morphogens Cell Lineages Patterning Spinal Cord Axon Navigation Barrier Function Ipsilateral Axons Crossing Commissural Axons Crossing Secretion Of Guidance Cues Attractants Repellents Guidance Receptors Signaling Modulation Investigating Properties Developmental Contexts Floor-Plate Conditioned Medium (FPcm) Axon Guidance
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