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Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
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Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Article DOI: 10.3791/50898-v • 11:22 min • February 24th, 2014
February 24th, 2014 •
1, 2, 3, 1, 1, 4, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology, Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

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Summary
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Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.

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Calcium Sparks Intact Skeletal Muscle Fibers Homeostatic Ca2+ Signaling Ryanodine Receptor (RyR) Sarcoplasmic Reticulum (SR) Intracellular Ca2+ Handling Resting Cardiomyocytes Resting Skeletal Muscle Fibers Experimental Protocol Flexor Digitorm Brevis (FDB) Muscle Fibers Fluorescent Ca2+ Indicators Laser Scanning Confocal Microscopy Hypoosmotic Stress Isotonic Physiological Solution Sarcolemmal Membrane Young Healthy FDB Muscle Fibers Dystrophic Skeletal Muscle Fibers Aged Skeletal Muscle Fibers Inositol (1,4,5)-triphosphate Receptor (IP3R)
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