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Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
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Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

Article DOI: 10.3791/51817-v • 06:46 min • September 4th, 2014
September 4th, 2014 •
1, 2
1Cell and Developmental Biology, University of Michigan, 2Department of Biosciences and Nutrition, Karolinska Instituet Novum

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Improved imaging technology is allowing three-dimensional imaging of organs during development. Here we describe a whole organ culture system that allows live imaging of the developing villi in the fetal mouse intestine.

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Mouse Fetal Whole Intestine Culture System Ex Vivo Manipulation Signaling Pathways Three-dimensional Live Imaging Villus Development Morphogenetic Processes Thin Sections Fixed Tissues Three-dimensional Information Serial Sections Tissue Orientation Developing Villi Intestinal Epithelial Cells Apical And Basal Surfaces Actin Cytoskeleton Intestinal Lumen Secondary Lumens Interkinetic Nuclear Migration Pseudostratified Epithelium
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