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JoVE Journal Neuroscience
Telomerase Activity in the Various Regions of Mouse Brain
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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Article DOI: 10.3791/51865-v • 10:14 min • September 2nd, 2014
September 2nd, 2014 •
1, 1, 1
1The Shraga Segal Department of Immunology, Microbiology & Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev

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Summary
Automatic Translation

Telomerase is expressed in the neonatal brain and also in distinct regions of the adult brain. We present a non-toxic time saving TRAP assay for the analysis of telomerase activity in various regions of the mouse brain and detection of differences in telomerase activity between male and female mouse brains.

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Telomerase Activity Mouse Brain Non-radioactive TRAP Assay Telomere Length Genomic Integrity Proliferation Lifespan Mitochondria Oxidative Stress Apoptosis Telomerase Activators Motor Neurons Lesions Male And Female Mouse Brain Age Dependence CHAPS Lysis Buffer Telomerase Substrate (TS Primer) PCR Reaction
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