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In vitro Methylation Assay to Study Protein Arginine Methylation
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In vitro Methylation Assay to Study Protein Arginine Methylation

Article DOI: 10.3791/51997-v • 10:01 min • October 5th, 2014
October 5th, 2014 •
1, 1, 1, 1, 1, 1,2, 1,3
1Department of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, 2Department of Hematology and Oncology, University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center

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Summary
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Protein arginine methylation, catalyzed by a class of enzymes viz., protein arginine methyl transferases (PRMTs), is the process of enzymatic addition of methyl group(s) to arginines within proteins. The in vitro methylation assay is the most dependable tool for assessing the methylation status of known or novel PRMT substrates.

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In Vitro Methylation Assay Protein Arginine Methylation Post-translational Modification Proteomic Approaches Immunoblotting Methyl-arginine Specific Antibodies PRMT Substrate Specificity Biochemical Assays Purified PRMTs Purified Substrate Radioisotope Labeled Methyl Donor Step-by-step Protocol Catalytically Active PRMT1 Ras-GTPase Activating Protein Binding Protein 1 (G3BP1) S-adenosyl-L-[methyl-3H] Methionine Physiological PRMT1 Substrates Mechanism Of Protein Arginine Methylation
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