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A lungo termine Time Lapse Imaging del mouse cocleari Espianti
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A lungo termine Time Lapse Imaging del mouse cocleari Espianti

Article DOI: 10.3791/52101-v • 10:43 min • November 2nd, 2014
November 2nd, 2014 •
1, 1,2,3
1Biological Sciences Platform, Sunnybrook Research Institute, 2Department of Otolaryngology - Head and Neck Surgery, University of Toronto, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Immagini dal vivo della coclea embrionale dei mammiferi è impegnativo, perché i processi di sviluppo a portata di mano operano su un gradiente temporale nell'arco di dieci giorni. Qui vi presentiamo un metodo per la coltura e quindi l'imaging embrionale dei tessuti espianto cocleare preso da un topo reporter fluorescente in cinque giorni.

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Bioingegneria live-immagini lasso di tempo coclea nell'orecchio topo giornalista lo sviluppo il microscopio incubatore Sox2
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