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Journal / Developmental Biology / Production and Use of Lentivirus to Selectively…
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Developmental Biology

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JoVE Journal Developmental Biology
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
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Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Article DOI: 10.3791/52179-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
1, 1, 1, 1,2, 1,2
1Department of Anatomy and Neuroscience, The University of Melbourne, 2The Florey Institute of Neuroscience and Mental Health Research, The University of Melbourne

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Summary
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Here we present protocols that offer a flexible and strategic foundation for virally manipulating oligodendrocyte precursor cells to overexpress proteins of interest in order to specifically interrogate their role in oligodendrocytes via the in vitro model of central nervous system myelination.

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Lentivirus Transduction Oligodendrocyte Precursor Cells In Vitro Myelination Assay Myelin Formation Glial Cells Central Nervous System Peripheral Nervous System Co-cultures Proteins Expression Lentiviral Infection Signaling Mechanisms Compensation Mechanisms Molecular Mechanisms
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