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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Article DOI: 10.3791/52211-v • 09:54 min • December 16th, 2014
December 16th, 2014 •
1, 1
1Division of Neuropathology, Department of Pathology, Pathobiology Graduate Program, Johns Hopkins School of Medicine

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This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.

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DNA Damage TUNEL Method Cell Death Detection DNA Breaks TdT Enzyme DUTP Labeling Fluorescence-based Labeling Semi-automated Quantitation Tissue Processing Autofluorescence Interference Signal-to-noise Ratio Apoptosis Detection Therapeutic Efficacy
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